mouse anti-mica/fitc流式抗體說(shuō)明書(shū)
catalog number : rs-0832r-fitc
quantity size : 0.1ml （dilute with ph 7.4 0.01 m pbs or antibody diluent ）
background: the mhc class i chain-related （mic） proteins are related to the major histocompatibility complex （mhc） class i proteins which are ubiquitously expressed and mediate the recognition of intracellular antigens by cytotoxic t cells. the mhc class i chain-related （mic） proteins are recognized by nkg2d, a receptor on nk and t cells, and promote anti-tumor activity. mica, a member of the mic family, is widely expressed on many tumors, and it is the mica/nkg2d interaction that is thought to stimulate the anti-tumor reactivity by t lymphocytes. mica is present in virtually every tissue except the nervous system, suggesting that mic protein expression may only be one component of the anti-tumor activity of the immune system.
function : seems to have no role in antigen presentation. acts as a stress-induced self-antigen that is recognized by gamma delta t-cells. ligand for the klrk1/nkg2d receptor. binding to klrk1 leads to cell lysis.
mica/fitc 流式抗體說(shuō)明書(shū)specificity : · anti-mica/fitc is a mouse monoclonal antibody unconjugated
· specific for mica/fitc of pig
· use for western blotting,elisa,immunoprecipitation and immunohistochemistry
· protein g affinity chromatography purification, purity :>95%
· isotype: igg
· mol wt: 43kda
application: ·flow-cyt=1:50-200
· if=1:50-200
· optimal working dilutions must be determined by the end user.
mica/fitc 流式抗體說(shuō)明書(shū)storage: store at –20 ºc for one year. avoid repeated freeze/thaw cycles. the lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºc. when reconstituted in sterile ph 7.4 0.01m pbs or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºc.
important note: this product as supplied is intended for research use only, not for use in human,therapeutic or diagnostic applications.